Part:BBa_K2622004

scFv_antiVGY

Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.

This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.

References

Pleckaityte, M., Mistiniene, E., Lasickiene, R., Zvirblis, G., & Zvirbliene, A. (2011). Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis. BMC biotechnology, 11, 100. https://doi.org/10.1186/1472-6750-11-100

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 41